Antioxidant properties of brown coal humic substances
- Details
- Category: Content №3 2024
- Last Updated on 28 June 2024
- Published on 30 November -0001
- Hits: 2613
Authors:
I.V.Efimova*, orcid.org/0000-0002-4374-2990, L.M.Litvinenko Institute of Physical Organic and Coal Chemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
O.V.Smirnova, orcid.org/0000-0003-4143-7535, L.M.Litvinenko Institute of Physical Organic and Coal Chemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
T.G.Shendrik, orcid.org/0000-0001-6629-6471, L.M.Litvinenko Institute of Physical Organic and Coal Chemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
* Corresponding author e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Naukovyi Visnyk Natsionalnoho Hirnychoho Universytetu. 2024, (3): 116 - 121
https://doi.org/10.33271/nvngu/2024-3/116
Abstract:
Humic substances (HS) belong to a fairly common class of organic compounds in nature, which are a component of soils, peat, lignite and sapropels. Some properties of humates (salts of humic acids) make it possible to use them in various fields – crop production, animal production, well drilling and soil structuring. The biological and chemical activity of humic substances is well known. It is caused by the presence of such functional groups as carboxyl, hydroxyl, carbonyl, nitrogen- and sulfur-containing in the composition of GR macromolecules. This fact also serves as a basis for a more extensive study of other properties of GR, namely antioxidant activity.
Purpose. Deepening and expanding the understanding of the biological and antioxidant activity of lignite humic (HA) and hymatomelanic (HmA) acids, determining its nature and evaluating the prospects of its use in little-studied processes of oxidation of organic and consumer substances.
Methodology. The paper used a photocolorimetric method for determining the antiradical activity (ARA) of two components of humic substances of domestic lignite - humic and hymatomelanic acids. The antioxidant activity (AOA) of humic and hymatomelanic acids from lignite was determined using the gas volumetric method, evaluating the rate of reaction inhibition using the example of the initiated liquid-phase oxidation of cumene with oxygen.
Findings. It was established that humic acids exhibit more pronounced antioxidant properties in the processes of radical-chain oxidation of hydrocarbons compared to hymatomelanic acids. The total AOA of HA is 1.68 relative units, which is almost three times higher than this figure for HmA (0.50). On the other hand, it has been experimentally proven that hymatomelanic acids have more pronounced antiradical properties than humic acids. ARA HmA is 0.90 relative units, which is approximately three times more than the similar value for HA (0.57).
Originality. Despite the fact that a number of methods for determining the antiradical and antioxidant activity of low-molecular antioxidants and their mixtures have been developed, a method for determining these indicators for high-molecular native compounds has not yet been created. For the first time, a method for determining the antiradical effect of humic substances using the stable 2.2-diphenyl-1-picrylhydrazyl radical (DPPH) was proposed and experimentally tested. A method for determining the antioxidant activity of humic and hymatomelanic acids in hydrocarbon oxidation processes (on the example of cumulus) has been developed. The convenience and satisfactory reproducibility of the developed methods are shown.
Practical value. The green-chemistry related development and application of drugs based on native and modified humic substances as polymers with adjustable redox properties (redox polymers) is a relevant direction of sustainable development of society. These substances are the basis for obtaining new medicines, plant protection products, new sorbents, preparations for the reclamation of territories contaminated with radionuclides, heavy metals, organic ecotoxicants, oil products, etc.
Keywords: antiradical activity, antioxidant activity, inhibitors, hymatomelanic acids, humic acids
References.
1. Lipczynska-Kochany, E. (2018). Humic substances, their microbial interactions and effects on biological transformations of organic pollutants in water and soil. Chemosphere, 202, 420-437. https://doi.org/10.1016/j.chemosphere.2018.03.104.
2. Mignone, R. A., Martin, M. V., Palazzi, V. I., de Mishima, B. L., & Martire, D. O. (2012). Modulation of Optical Properties of Dissolved Humic Substances by their Molecular Complexity. Photochem. Photobiol, 88, 792-800. https://doi.org/10.1111/j.1751-1097.2012.01135.x.
3. Trofimova, E. S., Zykova, M. V., Ligacheva, A. A., Sherstoboev, E. Y., Zhdanov, V. V., Belousov, M. V., …, & Dygai, A. M. (2016). Effects of humic acids isolated from peat of various origin on in vitro production of nitric oxide: A screening study. Bulletin of Experimental Biology and Medicine, 161, 687-692. https://doi.org/10.1007/s10517-017-3702-5.
4. Ivanushun, O. Ya., Yaremkevych, O. S., Semeniuk, I., Karpenko, О., Monka, N., & Lubenets, V. (2021). Antioxidant effect of humic acids on rat hepatocytes. Chemistry, technology and application of substances, 4, 64-71. https://doi.org/10.23939/ctas2021.02.064.
5. Buchko, O. M., Salyha, N. O., Svarchevska, O. Z., Maksymovych, I. Ia., & Senkiv, O. M. (2013). Immunological and hematological parameters of the blood of piglets under the action of humic additives. Bulletin of ONU, ser.: Biology, 18(3(32)), 73-81. https://doi.org/10.18524/2077-1746.2013.3(32).45283.
6. Buron, N., Porceddu, M., Roussel, C., Begriche, K., Trak-Smayra, V., Gicquel, T., Fromenty, B., & Borgne-Sanchez, A. (2017). Chronic and low exposure to a pharmaceutical cocktail induces mitochondrial dysfunction in liver and hyperglycemia: Differential responses between lean and obese mice. Environmental Toxicology, 32, 1375-1389. https://doi.org/10.1002/tox.22331.
7. Verrillo, M., Cozzolino, V., Spaccini, R., & Piccolo, A. (2021). Humic substances from green compost increase bioactivity and antibacterial properties of essential oils in Basil leaves. Chemical and Biological Technologies in Agriculture, 8, 1-14. https://doi.org/10.1186/s40538-021-00226-7.
8. Wu, Y., Zhou, S., Ye, X., Zhao, R., & Chen, D. (2011). Oxidation and coagulation removal of humic acid using Fenton process. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 379, 151-156. https://doi.org/10.1016/j.colsurfa.2010.11.057.
9. Cozzolino, V., Di Meo, V., Monda, H., Spaccini, R., & Piccolo, A. (2016). The molecular characteristics of compost afect plant growth, arbuscular mycorrhizal fungi, and soil microbial community composition. Biology and Fertility, 52, 15-29. https://doi.org/10.1007/s00374-015-1046-8.
10. Stepchenko, L. M., Dyomshyna, O., & Ushakova, H. (2021). Impact of biologically active additives of humic nature on the metabolic profile of broiler chickens. Achievements of Ukraine and the EU in ecology, biology, chemistry, geography and agricultural sciences, 262-288. https://doi.org/10.30525/978-9934-26-086-5-47.
11. Zykova, M. V., Schepetkin, I. A., Belousov, M. V., Krivoshchekov, S. V., Logvinova, L. A., Bratishko, K. A., …, & Quinn, M. T. (2018). Physicochemical Characterization and Antioxidant Activity of Humic Acids Isolated from Peat of Various Origins. Molecules (Basel, Switzerland), 23(4), 753. https://doi.org/10.3390/molecules23040753.
12. Horst, C., Sharma, V. K., Baum, J. C., & Sohn, M. (2013). Organic matter source discrimination by humic acid characterization: Synchronous scan fluorescence spectroscopy and Ferrate (VI). Chemosphere, 90, 2013-2019. https://doi.org/10.1016/j.chemosphere. 2012.10.076.
13. Schumacher, A., Vranken, T., Arts, C., & Habibovic, P. (2018). In vitro antimicrobial susceptibility testing methods: agar dilution to 3D tissue-engineered models. European Journal of Clinical Microbiology & Infectious Diseases, 37, 187-208. https://doi.org/10.1007/s10096-017-3089-2.
14. Canellas, L. P., & Olivares, F. L. (2014). Physiological responses to humic substances as plant growth promoter. Chemical and Biological Technologies in Agriculture, 1, 1186-1196. https://doi.org/10.1186/2196-5641-1-3.
15. Shang, E., Li, Y., Niu, J., Zhou, Y., Wang, T., & Crittenden, J. C. (2017). Relative importance of humic and fulvic acid on ROS generation, dissolution, and toxicity of sulfide nanoparticles. Water Research, 124, 595-604. https://doi.org/10.1016/j.watres.2017.08.001.
16. Kuznetsova, T. Y., Solovyova, N. V., Solovyov, V. V., & Kostenko, V. O. (2017). Antioxidant activity of melatonin and glutathione interacting with hydroxyl and superoxide anion radicals. The Ukrainian Biochemical Journal, 89(6), 22-30. https://doi.org/10.15407/ubj89. 06.022.
17. Aeschbacher, M., Graf, C., Schwarzenbach, R. P., & Sander, M. (2012). Antioxidant properties of humic substances. Environmental Science & Technology, 46, 4916-4925. https://doi.org/10.1021/es 300039h.
18. Shadyro, O., Samovich, S., Edimecheva, I., Novitsky, R., Khrutskin, V., Ihnatovich, L., ..., & Dubovik, B. (2021). Potential role of free-radical processes in biomolecules damage during COVID-19 and ways of their regulation. Free Radical Research, (just-accepted), 1-27. https://doi.org/10.1080/10715762.2021.1938024.
19. de Melo, B. A., Motta, F. L., & Santana, M. H. (2016). Humic acids: Structural properties and multiple functionalities for novel technological developments. Materials science & engineering. C, Materials for biological applications, 62, 967-974. https://doi.org/10.1016/j.msec.2015.12.001.
20. Efimova, I. V., Smirnova, O. V., Opeida, I. A., & Vakhitova, L. N. (2020). Effect of ascorbic acid and superoxide anion on the processes of inhibition of chain-radical oxidation in an aprotic medium. Issues of Chemistry and Chemical Technology, (4), 55-59. https://doi.org/10.32434/0321-4095-2020-131-4-55-59.
Newer news items:
- Automation of building an individual educational trajectory for a higher education student - 28/06/2024 21:21
- Financial determinants of the post-war reconstruction of the national economy on the principles of the European Green Deal - 28/06/2024 21:21
- Evaluation of the institutional development of innovative activities to ensure the economy of the state - 28/06/2024 21:21
- Personnel potential of industrial enterprises: formation and management - 28/06/2024 21:21
- Identification and suppression of signals of the rear lobe of the radiation pattern of the radar antenna - 28/06/2024 21:21
- Integration of the educational process in higher education with digital technologies - 28/06/2024 21:21
- Data flow management in information systems using blockchain technology - 28/06/2024 21:20
- Improvement of the method for optimization of predicting the efficiency of a robotic platform - 28/06/2024 21:20
- The environmental effectiveness of humate reagent in internal and external hydro-filling of quarries - 28/06/2024 21:20
- Integrated water resources monitoring system within the structure of environmental safety in southern Ukraine - 28/06/2024 21:20
Older news items:
- Current tools to control decarbonization in Ukraine both governmentally and locally - 28/06/2024 21:20
- Ecological and geochemical aspects of thermal effects on argillites of the Lviv-Volyn coal basin spoil tips - 28/06/2024 21:20
- Effect of hardened cement waste and fresh cement in the treatment of expansive soil - 28/06/2024 21:20
- Development of the concept for improving the management system of employee safety and health in Ukraine - 28/06/2024 21:20
- Rotor configuration for improved working characteristics of LSPMSM in mining applications - 28/06/2024 21:20
- Designing the functional surfaces of camshaft cams of internal combustion engines - 28/06/2024 21:20
- Endurance calculation of welded joints in tubbing erector mechanism using digital methods - 28/06/2024 21:20
- Effect of circumferential lean of pump-turbine runner blades on energy characteristics - 28/06/2024 21:20
- Influence of multiphase fuel injection on the technical and economic indicators of a transportation diesel engine - 28/06/2024 21:20
- Synthesis and research of the spatial eight-link mechanism of the barreling machine - 28/06/2024 21:20